Journal: iScience

Article Title: Gut bacteria induce IgA expression in pituitary hormone-secreting cells during aging

doi: 10.1016/j.isci.2023.107747

Figure Lengend Snippet:

Article Snippet: Multiplex Fluorescence v.2 kit , Advanced Cell Diagnostics , 323100.

Techniques: Virus, Recombinant, Red Blood Cell Lysis, Blocking Assay, Multiplex Assay, Fluorescence, Single Cell RNA-Seq, Software

Journal: Nature

Article Title: Astrocyte-neuron subproteomes and obsessive-compulsive disorder mechanisms.

doi: 10.1038/s41586-023-05927-7

Figure Lengend Snippet: Fig. 3 | SAPAP3 expression in striatal astrocytes. a, SAPAP3 protein abundance across the neuronal and astrocytic subcompartments. Not detected, SAPAP3 was not detected. Mean and s.e.m. are shown. b, Dlgap3 mRNA abundance measured in fragments per kilobase of exon per million, mapped fragments (FPKM) in neuronal or astrocyte RiboTag immunoprecipitation (IP). Mean and s.e.m. are shown. c, Left, uniform manifold approximation and projection (UMAP) plot of striatal cells (n = 31,956 individual cells, replotted from our published scRNA-seq data36. Middle, expression of Rbfox3 in neurons and Gja1 in the astrocytes. Right, violin plot showing the relative expression level of Dlgap3 per cell in neurons and astrocytes. Expression level is defined as the log2 normalized gene count per cell. OPC, oligodendrocyte precursor cell. d, Representative image of the dorsal striatum (dStr) from either WT or SAPAP3 KO mice labelled by RNAscope in situ hybridization for Dlgap3 mRNA (purple)

Article Snippet: Dual ISH-IHC was performed using a Multiplex RNAscope (v.2) with integrated co-detection work flow (ACDBio 323180 and 323110).

Techniques: Expressing, Quantitative Proteomics, Immunoprecipitation, RNAscope, In Situ Hybridization